The duo, along with puppet Kate Monster, will perform "The More You Ruv Someone" and Larsen will also sing the first-act finale, "There's a Fine, Fine Line."
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As previously announced, Avenue Q will play its final performance on Broadway at the John Golden Theatre Sept. 13, having played 2,534 performances and 22 previews.
Avenue Q features music and lyrics by Robert Lopez and Jeff Marx, a book by Jeff Whitty and direction by Jason Moore. The musical is produced by Kevin McCollum, Robyn Goodman, Jeffrey Seller, The Vineyard Theatre and The New Group.
Puppets were conceived and designed by original cast member Rick Lyon. The production has musical supervision by Stephen Oremus, choreography by Ken Roberson, scenic design by Anna Louizos, costume design by Mirena Rada, lighting design by Howell Binkley and sound design by Acme Sound Partners.
For more information visit www.AvenueQ.com.