The 11:30 PM late-night show will also feature Hannah Shankman, Charlie Rosen and Asmeret Ghebremichael.
The Skivvies are known for their acoustic performances of pop songs, wearing nothing but their underwear.
Lauren Molina, Nick Cearley and their guests play instruments ranging from the cello, ukulele, guitar and glockenspiel to the melodica.
Tickets are priced $30-$60. There is a $25 food/beverage minimum.
54 Below is located at 254 W. 54th Street.
For more information visit 54Below.com.