Directed by John Thomas Cecil, the six-person cast includes Casey Keelen, Zach DeSutter, Christy Gallo, Megan Hollenbeck, Sanford Knox Jr. and Jeremy Wilkins.
Prior to the record-breaking attempt, The Bald Soprano will also be presented Oct. 18-20.
Eugene Ionesco's absurdist comedy, which was written when he was learning the English language, was first performed in 1950.
If the cast completes the 30-hour performance, they would break the previous record of 23 hours, 33 minutes, 54 seconds set by the 27 O'Clock Players in Belmar, NJ, in 2010.
More information can be found by visiting ensembletheatreofchattanooga.com.