The Broadway actress and "Glee" star will perform "America the Beautiful" during the pregame portion of the annual event, according to E! Online.
Entertainment at the Super Bowl will also include Christina Aguilera singing the "National Anthem" and the Black Eyed Peas performing during the halftime show.
The Bridgestone Super Bowl XLV will air on the Fox network Feb. 6. A new episode of "Glee" will follow.
Michele has appeared on Broadway in Ragtime, Les Miserables and Spring Awakening.