Criss, who also topped last year's list of the "100 hottest men on Earth," is joined by "Glee" co-stars Chris Colfer (this year's runner up), Mark Salling, Matthew Morrison, Cory Monteith, Jonathan Groff, Max Adler, Chord Overstreet, Grant Gustin and Harry Shum Jr.

Other notables among the sixth annual "Hot 100" include Wilson Cruz (Rent), Justin Bartha (Lend Me a Tenor), Andrew Garfield (Death of a Salesman), Daniel Radcliffe (How to Succeed…), Hugh Jackman (The Boy From Oz), Cheyenne Jackson (Xanadu), David Burtka (Gypsy), Benedict Cumberbatch (Frankenstein), Zac Efron ("High School Musical"), Anderson Cooper (How to Succeed…), Ricky Martin (Evita), Adam Lambert (Wicked), Neil Patrick Harris (Assassins), Matt Bomer (8), John Barrowman (Putting It Together), Bradley Cooper (Three Days Rain), Luke MacFarlane (The Normal Heart), Russell Tovey (The History Boys), Jon Hamm, Alex Pettyfer, Damon Wayans Jr., President Barack Obama, Brad Pitt, Chace Crawford, Joe Manganiello, Gale Harold, Ewan McGregor, Ian Somerhalder and David Tennant.

Criss, who made his Broadway debut as J. Pierrepont Finch in How to Succeed…, plays the role of Blaine, the openly-gay Warbler at Dalton Academy, on Fox's "Glee." Prior to "Glee," Criss was best known for his portrayal of Harry Potter in the YouTube hit, "A Very Potter Musical."

For the complete list, visit AfterElton.com.