The evening, which takes audiences on a journey through the life of late film and stage actor Stewart ("Harvey," "It's a Wonderful Life"), is billing the Las Vegas run as a pre-Broadway engagement.
It will play the Las Vegas Hotel's Shimmer Cabaret through April 3.
Jimmy Stewart & Friends also promises Little's impressions of Cary Grant, George Burns, Ronald Reagan, Katherine Hepburn and John Wayne.
Little is known for appearances on "Laugh-In," "The Tonight Show," "Hollywood Squares," "KopyKats," "The Julie Andrews Hour" and his 1970's series "The Rich Little Show."