The special, taped at the Biltmore Hotel in downtown Los Angeles, includes musical performances and surprise appearances, as well as a trip down memory lane with White.
Celebrities in attendance include Mary Tyler Moore, Carol Burnett, William Shatner, Hugh Jackman, Jay Leno, Amy Poehler and Joel McHale.
White is also joined by her "Mary Tyler Moore Show" co-stars Ed Asner, Valerie Harper and Gavin MacLeod and "Hot in Cleveland" ladies Valerie Bertinelli, Wendie Malick and Jane Leeves.
Following "Betty White's 90th Birthday" is a sneak preview of White's hidden-camera reality series "Betty White's Off Their Rockers."
White won Emmy Awards for her work on "The Mary Tyler Moore Show," "The Golden Girls," "The John Larroquette Show," "Saturday Night Live" and a Daytime Emmy for "Just Men!"