The entire "Glee" cast will perform on the show and sit down with the talk show goddess. Series creator Ryan Murphy will also be on hand for the episode that will feature backstage video from the show.
In anticipation of the "Glee" themed episode, Oprah.com plans to launch a page with character guides and behind the scenes photo galleries.
New episodes of "Glee," which stars Broadway's Lea Michele and Matthew Morrison, will resume on FOX April 13.