Tom Wojtunik, who penned the evening, will direct with music direction by Justin Fischer.
York alumni assembled for the concert include Klea Blackhurst, Liam Forde, Josh Grisetti, Mark Nadler, Debra Walton, Zakiya Young, Christine Baird, Erin Eichfeld, Kevin Lagasse, Justin Luciano, Zakk Mannella and MacKenzie Perpich.
The program will be a sampler of songs from Main Stage productions presented over the past 45 years, including The Golden Apple, Enter Laughing, Closer Than Ever, Yank!, Storyville and nine Stephen Sondheim musicals.
A second anniversary concert is scheduled for Jan. 19, 2015.
54 Below is located at 254 West 54th Street. There is a $40 cover charge and $25 food and beverage minimum. Tickets and information are available at www.54Below.com.