The production, which will be staged at the Castillo Theater, will mark the first time any work by Beckett has been performed in Yiddish, according to the New Yiddish Rep.
Performances begin Sept. 20 and will continue through Oct. 13.
The work has been translated into Yiddish from the French and English versions by Shane Baker, who will also star in the production. It will be staged with English and Russian supertitles.
Directed by Moshe Yassur, the cast will also include Rafael Goldwasser, Avi Hoffman, Nicholas Jenkins and David Mandelbaum.
Waiting for Godot first premiered in 1953, and Beckett received the Nobel Prize in Literature for the absurdist play, which follows two characters, Vladimir and Estragon, as they wait endlessly and in vain for the arrival of someone named Godot. Tickets and more information can be found by visiting newyiddishrep.org.