Pasquale, who has signed on for a recurring role, will play Luke Granby, Reagan's (Christina Applegate) new boss and a potential love interest for Maya Rudolph's character, Ava.
The NBC series stars Applegate (Sweet Charity) as a new mom who returns to work with the support of her stay-at-home husband, Chris (Will Arnett).
Pasquale has appeared on Broadway in Reasons to Be Pretty. Most recently, he was seen in the Off-Broadway production of Tony Kushner's The Intelligent Homosexual's Guide to Capitalism and Socialism With a Key to the Scriptures. Pasquale was nominated for Outer Critics Circle and Drama Desk awards for his performance in Off-Broadway's A Man of No Importance. His television credits include "Rescue Me" and "Playboy Club."
"Up All Night" airs Thursday nights at 9:30 PM ET on NBC.